产品货号：
GS4454
中文名称：
重组人FGF-21
英文名称：
Recombinant Human Fibroblast Growth Factor-21
产品规格：
5μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 19.4kDa,a single non-glycosylated polypeptide chain containing 181 amino acids.
储存条件：冷冻（-20℃）
概述
Fibroblast growth factor-21(FGF-21)belongs to the large FGF family which is encoded by the FGF-21 gene and it is specifically induced by HMGCS2 activity.All FGF family members are heparin binding growth factors with a core 120 amino acid(a.a.)FGF domain that allows for a common tertiary structure and they are involved in a variety of biological processes including embryonic development,cell growth,morphogenesis,tissue repair,tumor growth and invasion.FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression(but not SLC2A4/GLUT4 expression)and the activity depends on the presence of KLB.FGF-21 contains a 28 a.a.signal sequence and a 181 a.a.mature region but show limited binding to heparin.In addition,Mature human FGF-21 respectively shows 81%a.a.identity to murine and rat FGF-21,and is known to be active on murine cells.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组人FGF-21，FGF-21, Human，Recombinant Human Fibroblast Growth Factor-21
储存条件：冷冻（-20℃）
概述
Fibroblast growth factor-21(FGF-21)belongs to the large FGF family which is encoded by the FGF-21 gene and it is specifically induced by HMGCS2 activity.All FGF family members are heparin binding growth factors with a core 120 amino acid(a.a.)FGF domain that allows for a common tertiary structure and they are involved in a variety of biological processes including embryonic development,cell growth,morphogenesis,tissue repair,tumor growth and invasion.FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression(but not SLC2A4/GLUT4 expression)and the activity depends on the presence of KLB.FGF-21 contains a 28 a.a.signal sequence and a 181 a.a.mature region but show limited binding to heparin.In addition,Mature human FGF-21 respectively shows 81%a.a.identity to murine and rat FGF-21,and is known to be active on murine cells.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞96%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5μg/ml,corresponding to a specific activity of>2.0×103 IU/mg in the presence of 5μg/ml of rMuKlotho-β and 10 μg/ml of heparin. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4. |
| 内毒素 | Less than 1 EU/μg of rHuFGF-21 as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S |
相关搜索：重组人FGF-21，FGF-21, Human，Recombinant Human Fibroblast Growth Factor-21